The one-off game at the Accra Sports stadium is crucial to the chances of both teams qualifying for the next stage of the competition.
Hearts are making a return to the Champions League for the first time in about a decade after winning the domestic double in the recently ended Ghana football season.
They enter this game high on confidence but must play well to overcome their opponents.
Hearts of Oak's opponents, CI Kamsar are powerhouses in Guinea and had some experience in continental battles, playing in the CAF Confederation Cup last season.
The two teams were set to play the first leg in Guinea but a coup d'etat forced CAF to make the game a one-legged tie.
If the game ends in a draw, we will move straight to penalties to decide the winner.
Meanwhile, the game will be played behind closed doors.
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